Beyond Efficacy: High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts

Pedro Akharenegbe; Aleruchi  Chuku; Maryam Isah; Hannah Eyo  Nsemoh; Bashiru Eya  Sani; Amina Ladidi  Musa; Isah Ibrahim  Okposhi

doi:10.62050/ljsir2026.v4n1.730

Beyond Efficacy

High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts

المؤلفون

Pedro Akharenegbe
pedroakharenegbe@gmail.com

Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
Aleruchi Chuku
Department of Microbiology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
Maryam Isah
Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
https://orcid.org/0000-0001-8728-4090
Hannah Eyo Nsemoh
Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
Bashiru Eya Sani
Department of Microbiology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
https://orcid.org/0000-0002-2983-3861
Amina Ladidi Musa
Department of Biotechnology, Federal University of Lokoja, PMB 1154, Kogi State, Nigeria
Isah Ibrahim Okposhi
Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria

الكلمات المفتاحية:

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الملخص

Aflatoxin B1 (AFB1) contamination in staple crops is a major cause of hepatocellular carcinoma in sub-Saharan Africa. Although plant extracts like Azadirachta indica (neem) and Curcuma longa (turmeric) are proposed as low-cost detoxificants, their in vivo efficacy and safety profiles are inadequately characterized. This study first quantified aflatoxin contamination in 300 stored groundnut samples from Nasarawa State, Nigeria, using High-Performance Liquid Chromatography (HPLC). Concurrently, the detoxification potential and toxicity of aqueous extracts of these botanicals were evaluated in an albino rat model. Rats were divided into groups and treated via oral gavage for 28 days with AFB1 alone or in combination with either C. longa or low- (1000 mg/kg) or high-dose (5000 mg/kg) A. indica extract. Serum biochemistry was analyzed using standard diagnostic kits. The groundnut analysis revealed widespread contamination, with 51.3% of samples exceeding the USFDA safety limit (20 µg/kg) and AFB1 levels as high as 1791 µg/kg. In the rat model, high-dose A. indica (5000 mg/kg) significantly elevated serum alanine aminotransferase (92.56 vs. 76.23 IU/L in controls, P < 0.003) and reduced total protein, indicating hepatotoxicity. C. longa showed no significant biochemical alterations. These findings highlight a serious public health risk from aflatoxin exposure and reveal a dose-dependent hepatotoxicity that severely limits the therapeutic use of high-dose neem extract. The promotion of plant-based detoxification requires caution, underscoring the urgent need to improve post-harvest storage infrastructure to mitigate contamination at its source.

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منشور

2026-02-17

كيفية الاقتباس

Beyond Efficacy: High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts. (2026). Lafia Journal of Scientific and Industrial Research, 4(1), 75-80. https://doi.org/10.62050/ljsir2026.v4n1.730