Hand Carriage of Microorganisms by Students of Federal University of Lafia, Nasarawa State, Nigeria

Authors

  • Peter Uteh Upla Department of Microbiology, Federal University of Lafia Author
  • Bashiru Eya Sani Department of Microbiology, Federal University of Lafia Author https://orcid.org/0000-0002-2983-3861
  • Osuyi Gerard Uyi Department of Microbiology, Federal University of Lafia Author
  • Igoche Naomi Ibe Department of Microbiology, Federal University of Lafia Author
  • Salamatu Idris Department of Microbiology, Federal University of Lafia Author
  • Oluwatosin Okunade Department of Microbiology, Federal University of Lafia Author
  • Naja'atu Shehu Hadi Department of Microbiology, Federal University of Lafia Author
  • Abdulaziz Aliyu Dardau Department of Microbiology, Federal University of Lafia Author
  • Kabiru Shuaibu Department of Microbiology, Federal University of Lafia Author
  • Fatima Yusuf Al-Mustapha Department of Microbiology, Federal University of Lafia Author
  • Gladys Abel Angbalaga Department of Microbiology, Federal University of Lafia Author

DOI:

https://doi.org/10.62050/ljsir2023.v1n2.234

Keywords:

Flora, Hands, Student, Transient, Palm, Nails

Abstract

Microbes from the body's regular flora and transient microorganisms from the environment are found on human hands. Hands can also be used to spread disease from one person to another, especially among close persons. This study aimed to isolate and identify microorganisms from students' hands and assess the occurrence of these bacteria based on gender, level of study, faculty, and hand area (palm and nails swab). Using the pour plate method, a total of sixty (60) hand swab samples (thirty (30) from both palm and nails) were collected and tested for bacterial and fungal presence. Bacteria isolated were Staphylococcus epidermidis (80.00 %), Staphylococcus aureus (75.00 %), Enterococcus spp (50.00 %), Micrococcus spp (46.67 %), Escherichia coli (45.00 %), Klebsiella spp (45.00 %), Bacillus spp (30.00 %), Salmonella spp (13.33 %) and Streptococcus spp (10.00 %). The fungi isolated were Aspergillus niger (45.00 %), Penicillium spp (23.33 %), Mucor spp (21.67 %), Candida spp (20.00 %) and Saccharomyces spp (15.00 %).  Gender, level of study, faculty, and area of hand swab revealed no statistically significant variation in the presence of numerous bacterial and fungal species at p<0.05. These findings revealed that the microbial burden on students' hands was significant and was unaffected by gender, level of study, or faculty. To improve students' overall health, appropriate hygiene, including regular handwashing practice, and public education about the importance of hands in disease transmission should be supported.

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References

Grice, E. and Segre, J. (2012). The human microbiome: our second genome. Annual Review of Genomics Human Genetics, 13, 151–170. https://doi.org/10.1146%2Fannurev-genom-090711-163814

Hentages, D. J. (1993). The anaerobic microflora of the human body. Clinical Infectious Disease, 16(14), 175-180. https://doi.org/10.1093/clinids/16.supplement_4.s175

Dethlefsen, L., McFall-Ngai, M. and Relman, D. A. (2007). An ecological and evolutionary perspective on human–microbe mutualism and disease. Nature, 449, 811-818. https://doi.org/10.1038/nature06245

Prescott, L. M., Harley, J. P. and Clem, D. A. (2005). Microbiology. McGraw Hill Company Incorporated, New York, 6, 960

Leake, S. L. (2013). Is human DNA enough? Potential for bacterial DNA. Frontiers in Genetics, 4, 1–3. https://doi.org/10.3389/fgene.2013.00282

Noble, W. C. and Somerville, D. A. (1997). Microbiology of Human Skin. Philadelphia: WB Saunders

Costello, E. K. Lauber, C. L., Hamady, M., Fierer, N., Gordon, J. I. and Knight, R. (2009). Bacterial community variation in human body habitats across space and time. Science, 326, 1694–1697. https://doi.org/10.1126/science.1177486

Grice, E., Kong, H., Conlan, S., Deming, C., Davis, J., Young, A., Bouffard, G., Blakesley, R., Murray, P., Green, E., Turner, M. and Segre, J. (2009). Topographical and temporal diversity of the human skin microbiome. Science, 324, 1190–1192. https://doi.org/10.1126%2Fscience.1171700

SanMiguel, A. and Grove, E. A. (2015). Interactions between host factors and the skin microbiome. Cellular and Molecular Life Science, 72(8), 1499–1515. https://doi.org/10.1007/s00018-014-1812-z

Nobel, W. C. (1993). The skin microflora and microbial skin disease, Cambridge University Press, Cambridge, United Kingdom

Watutantrige, R., Premalatha, P., Lum, W. and Evelyn, C. (2012). A study on hand contamination and hand washing practices among medical students. International Scholarly Research Notices, 1-5. https://doi.org/10.5402/2012/251483

Musa, E. K., Desai, N. and Casewell, M. N. (1990). The survival of Acinetobacter calcoaceticus inoculated on fingertips and formica. Journal of Hospital Infection, 15, 219-217. https://doi.org/10.1016/0195-6701(90)90029-n

Fryklund, B., Tullus, K. and Burman, L. G. (1995). Survival on skin and surfaces of epidemic and on epidemic strains of Enterobacteria from neonatal special care units. Journal of Hospital Infection, 29, 210-208. https://doi.org/10.1016/0195-6701(95)90329-1

Maji, J. W., Sandra, A. L. and Danladi, M. D. (2018). Comparative Studies on the Bacteria associated with hands of School pupils in government and private primary schools in Dutsinma, Kastina State. Archives of Clinical Microbiology, 9(4), 87. http://dx.doi.org/10.4172/1989-8436.100087

Dodrill, L., Schmidt, W. P., Cobb, E., Donachie, P., Curtis, V. and De-Barra, M. (2011). The effect of hand washing with water or soap on bacterial contamination of hands. Int. Sci. J. on Envtal Resource Public Health, 8, 97-104. https://doi.org/10.3390/ijerph8010097

Lindberg, E., Adlerberth, I., Hesselmar, B., Saalman, R. and Strannegared, A. (2004). High rate of transfer of Staphylococcus aureus from parental skin to infect gut flora. Journal of Clinical Microbiology, 42, 530-534. https://doi.org/10.1128/jcm.42.2.530-534.2004

De Alwis, W. R., Pakirisamy, P., San, L. W. and Xiaofen, E. C. (2012). A study on hand contamination and Hand Washing practices among medical students. International School Research Network, 2012, 1-5. https://doi.org/10.5402/2012/251483

Akharenegbe, P., Chuku, A., Mawak, J., Sani, B., Nsemoh, H. E. and Fadayomi, V. K. (2022). Fungal biodiversity associated with groundnuts stored in Nasarawa State12. GSC Biological and Pharmaceutical Sciences, 18(03), 023–029. https://doi.org/10.30574/gscbps.2022.18.3.0087

Chuku, A., Arikpo, G., Obande, G., Akharenegbe, P., Uteh, P. U. and Namang, M. (2016). A mycological assessment of the air quality in flood-prone homes within Lafia Local Government Area of Nasarawa State. FULafia Journal of Science and Technology, 2, 1-10

Aiello, A. E., Marshall, B., Levy, S. B., Della-Latta, P. and Larson, E. (2004). Relationship between Triclosan and susceptibilities of bacteria isolated from hands in the community. Antimicro. Agents and Chemother., 48(8), 2973-2979. https://doi.org/10.1128/aac.48.8.2973-2979.2004

Ichor, T., Aondoakaa, E. M. and Ebah, E. E. (2018). Comparative studies on the antibacterial activity of alcohol-based hand sanitizers against bacteria isolates from the hands of undergraduate students of University of Agriculture, Makurdi. J. Clin. Case Reports, 8, 7. http://dx.doi.org/10.4172/2165-7920.10001143

Oniya, M. O., Obajuluwa, S. E., Alade, E. T. and Oyewole, O. A. (2006). Evaluation of microorganisms transmissible through handshake. African Journal of Biotechnology, 5(11), 118-112

Oranusi, S., Dahunsi, S. O., Owoso, O.O. and Olatile, T. (2013). Microbial profiles of hands, foods, easy contact surfaces and food contact surfaces: A case study of a University Campus. Novus International Journal of Biotechnology & Bioscience, 2(1), 30-38

Tambekar, D. H., Shirsat, S. D., Kakde S. R. and Ambekar, K. B. (2009). Hand hygiene and health: An epidemiological study of students in Amravati. Afri. J. of Infecti. Diseases, 3(1), 26-30. http://dx.doi.org/10.4314/ajid.v3i1.55076

Oranusi, S. and Braide, W. A. (2012). Study of microbial safety of ready-to-eat foods vended on highways: Onitsha-Owerri, South East Nigeria. Int. Res. J. of Microbio., 3(2), 66-71

Oranusi, S. and Olorunfemi, O. J. (2011). Microbiological safety evaluation of street vended ready-to-eat fruits sold in Ota, Ogun state, Nigeria. International Journal of Research in Biological Sciences, 1(3), 22-26 25

Oranusi, S., Galadima, M. and Umoh, V. J. (2006). Toxicity test and bacteriophage typing of Staphylococcus aureus isolates from food contact surfaces and foods prepared by families in Zaria, Nigeria. African Journal of Biotechnology, 5 (4), 362-365

Hedderwick, S. A., McNeil, S. A., Lyons, M. J. and Kauffman, C. A. (2000). Pathogenic organisms associated with artificial fingernails worn by healthcare workers. Infection Control and Hospital Epidemiology, 21, 505-509. https://doi.org/10.1086/501794

Lin, C. F., Wu, M., Doyle, P. and Williams, K. (2002). Poster Presentation. International Association for Food Protection Annual Meeting, an Diego, CA

Cruz, J. P., Cruz, C. P., Suleiman, A. and Al-Otaibi, D. (2015). Gender differences in hand hygiene among Saudi nursing students. Int. Journal of Infection Control, 11, 4. https://doi.org/10.3396/IJIC.v11i4.029.15

Halder, A. K., Tronchet, C. and Akhter, S. (2010). Observed hand cleanliness and other measures of hand washing behavior in rural Bangladesh. Biomed Central Public Health, 10, 545. https://doi.org/10.1186/1471-2458-10-545

Snelling, A. M., Kerr, K. G. and Heritage, J. (1991). The survival of Listeria monocytogens on fingertips and factors affecting elimination of the organism by hand washing and disinfection. J. of Food Protec., 54(5), 343-348. https://doi.org/10.4315/0362-028x-54.5.343

Taylor, J. J., Basco, R., Zaied, A. and Ward, C. (2010). Hand hygiene knowledge of college students. Journal of the American Society for Medical Technology, 23(2), 89-93

Finley, R. L., Collignon, P. and Larsson, D. G. J. (2013). The scourge of antibiotic resistance: the important role of the environment. Clinical Infectious Disease, 57, 704–710. https://doi.org/10.1093/cid/cit355

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Published

2023-10-31

How to Cite

Hand Carriage of Microorganisms by Students of Federal University of Lafia, Nasarawa State, Nigeria. (2023). Lafia Journal of Scientific and Industrial Research, 1(1 &amp; 2), 22-27. https://doi.org/10.62050/ljsir2023.v1n2.234