Beyond Efficacy

High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts

Authors

  • Pedro Akharenegbe
    Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
  • Aleruchi Chuku
    Department of Microbiology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
  • Maryam Isah
    Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
    https://orcid.org/0000-0001-8728-4090
  • Hannah Eyo Nsemoh
    Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
  • Bashiru Eya Sani
    Department of Microbiology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria
    https://orcid.org/0000-0002-2983-3861
  • Amina Ladidi Musa
    Department of Biotechnology, Federal University of Lokoja, PMB 1154, Kogi State, Nigeria
  • Isah Ibrahim Okposhi
    Department of Science Laboratory Technology, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria

Keywords:

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Abstract

Aflatoxin B1 (AFB1) contamination in staple crops is a major cause of hepatocellular carcinoma in sub-Saharan Africa. Although plant extracts like Azadirachta indica (neem) and Curcuma longa (turmeric) are proposed as low-cost detoxificants, their in vivo efficacy and safety profiles are inadequately characterized. This study first quantified aflatoxin contamination in 300 stored groundnut samples from Nasarawa State, Nigeria, using High-Performance Liquid Chromatography (HPLC). Concurrently, the detoxification potential and toxicity of aqueous extracts of these botanicals were evaluated in an albino rat model. Rats were divided into groups and treated via oral gavage for 28 days with AFB1 alone or in combination with either C. longa or low- (1000 mg/kg) or high-dose (5000 mg/kg) A. indica extract. Serum biochemistry was analyzed using standard diagnostic kits. The groundnut analysis revealed widespread contamination, with 51.3% of samples exceeding the USFDA safety limit (20 µg/kg) and AFB1 levels as high as 1791 µg/kg. In the rat model, high-dose A. indica (5000 mg/kg) significantly elevated serum alanine aminotransferase (92.56 vs. 76.23 IU/L in controls, P < 0.003) and reduced total protein, indicating hepatotoxicity. C. longa showed no significant biochemical alterations. These findings highlight a serious public health risk from aflatoxin exposure and reveal a dose-dependent hepatotoxicity that severely limits the therapeutic use of high-dose neem extract. The promotion of plant-based detoxification requires caution, underscoring the urgent need to improve post-harvest storage infrastructure to mitigate contamination at its source.

Dimensions

Kumar, P., Mahato, D. K., Kamle, M., Mohanta, T. K., & Kang, S. G. (2017). Aflatoxins: A global concern for food safety, human health, and their management. Frontiers in Microbiology, 8, 2170. https://doi.org/10.3389/fmicb.2017.02170

Wild, C. P., & Gong, Y. Y. (2010). Mycotoxins and human disease: A largely ignored global health issue. Carcinogenesis, 31(1), 71–82. https://doi.org/10.1093/carcin/bgp264

Waliyar, F., Umeh, V. C., Chabi, M., Traore, A., Osiru, M., Ntare, B. R., & Diarra, B. (2015). Aflatoxin contamination in groundnut: A concern for African agriculture. Journal of Agricultural Science, 153(3), 201–213. https://doi.org/10.1017/S0021859614000394

Wu, F. (2008). A tale of two commodities: How EU mycotoxin regulations have affected US tree nut industries. World Mycotoxin Journal, 1(1), 95–102. https://doi.org/10.3920/WMJ2008.x006

Mutegi, C. K., Wagacha, J. M., Kimani, J., Otieno, G., Wanyama, R., Hell, K., & Kimenju, S. C. (2013). Prevalence and mitigation of aflatoxins in Kenya (1960–2020). World Mycotoxin Journal, 6(3), 241–254. https://doi.org/10.3920/WMJ2012.1500

Subapriya, R., & Nagini, S. (2005). Medicinal properties of neem leaves: A review. *Current Medicinal Chemistry - Anti-Cancer Agents, 5*(2), 149–156. https://doi.org/10.2174/1568011053174828

Asghar, H. A., Abbas, S. Q., Arshad, M. K., Jabin, A., Usman, B., Aslam, M., & Asghar, A. (2022). Therapeutic potential of Azadirachta indica (Neem) - A comprehensive review. Scholars International Journal of Traditional and Complementary Medicine, 5(3), 47-64.

Biswas, K., Chattopadhyay, I., Banerjee, R. K., & Bandyopadhyay, U. (2002). Biological activities and medicinal properties of neem (Azadirachta indica). Current Science, 82(11), 1336–1345.

Yamane, T. (1967). Statistics: An Introductory Analysis (2nd ed.). Harper and Row. ISBN: 978-0060473134

National Institutes of Health (NIH). (2011). Guide for the Care and Use of Laboratory Animals (8th ed.). National Academies Press. https://doi.org/10.17226/12910

Balan, B., Dhaulaniya, A. S., Kumar, M. M., & Kumar, P. (2024). Aflatoxins in Food: Prevalence, Health Effects, and Emerging Trends in Its Mitigation An Updated Review. Food Safety and Health, 2(1), 39–71. https://doi.org/10.1002/fsh3.12030

Chiewchan, N., Mujumdar, A. S., & Devahastin, S. (2015). Application of Drying Technology to Control Aflatoxins in Foods and Feeds: A Review. Drying Technology, 33(12), 1700-1712. https://doi.org/10.1080/07373937.2015.1068795

Smigic, N., Tomic, N., Udovicki, B., Djekic, I., & Rajkovic, A. (2022). Prevention and practical strategies to control mycotoxins in the wheat and maize chain. Food Control, 136, 108855. https://doi.org/10.1016/j.foodcont.2022.108855

Akharenegbe, P., Isah, M., Nsemoh, H. E., Jayeoba, G., & Ibrahim, O. I. (2025). Prevalence and environmental determinants of aflatoxin contamination in maize sold in open markets across Nasarawa State, Nigeria. Lafia Journal of Scientific and Industrial Research, 3(1), 66–73. https://doi.org/10.62050/ljsir2025.v3n1.391

Gizachew, D., Chang, C. H., Szonyi, B., De La Torre, S., & Ting, W. E. (2019). Aflatoxin B1 (AFB1) production by Aspergillus flavus and Aspergillus parasiticus on ground Nyjer seeds: The effect of water activity and temperature. International Journal of Food Microbiology, 296, 8–13. https://doi.org/10.1016/j.ijfoodmicro.2019.02.017

Muga, F. C., Marenya, M. O., & Workneh, T. S. (2019). Effect of temperature, relative humidity and moisture on aflatoxin contamination of stored maize kernels. Bulgarian Journal of Agricultural Science, 25(2), 271–277.

Nwobodo, E. I., Nwosu, D. C., Ogbodo, S. O., Ugwuene, F. O., Ihim, A. C., Ani, N. O., Nnodim, J. K., & Ani, O. (2018). Effects of Azadirachta indica leaf aqueous extract on the antioxidant enzymes in paracetamol-induced hepatotoxicity in Wistar rats. International Journal of Biological and Chemical Sciences, 12(1), 1–10. https://doi.org/10.4314/ijbcs.v12i1.1

Ashraf, K., Mujeeb, M., & Ahmad, A. (2018). A comprehensive review on phytochemical and pharmacological aspects of Curcuma longa Linn. Journal of Pharmaceutical Research International, 22(6), 1-17. https://doi.org/10.9734/JPRI/2018/46143

cover

Published

2026-02-17

How to Cite

Beyond Efficacy: High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts. (2026). Lafia Journal of Scientific and Industrial Research, 4(1), 75-80. https://doi.org/10.62050/ljsir2026.v4n1.730

Issue

Volume 4, Issue 1 (April, 2026), Lafia Journal of Scientific and Industrial Research (LJSIR)

Section

Life Sciences
Copyright & Licensing

Copyright (c) 2026 Pedro Akharenegbe, Aleruchi Chuku, Maryam Isah, Hannah Eyo Nsemoh, Bashiru Eya Sani, Amina Ladidi Musa, Isah Ibrahim Okposhi (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Beyond Efficacy: High-Dose Neem Extract Induces Hepatotoxicity in Rats amidst Widespread Aflatoxin Contamination of Nigerian Groundnuts. (2026). Lafia Journal of Scientific and Industrial Research, 4(1), 75-80. https://doi.org/10.62050/ljsir2026.v4n1.730

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