Prevalence and Environmental Determinants of Aflatoxin Contamination in Maize Sold in Open Markets Across Nasarawa State, Nigeria

Pedro Akharenegbe; Maryam Isah; Hannah Eyo Nsemoh; Glory Jayeoba; Okposhi Isah Ibrahim

doi:10.62050/ljsir2025.v3n1.391

Prevalence and Environmental Determinants of Aflatoxin Contamination in Maize Sold in Open Markets Across Nasarawa State, Nigeria

Authors

Pedro Akharenegbe
Pedroakharenegbe@gmail.com

Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa, Nigeria
Maryam Isah
Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa, Nigeria
https://orcid.org/0000-0001-8728-4090
Hannah Eyo Nsemoh
Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa, Nigeria
Glory Jayeoba
Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa, Nigeria
Okposhi Isah Ibrahim
Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa, Nigeria

Keywords:

Aflatoxin , maize , Aspergillus flavus , contamination , Nasarawa State

Abstract

This study evaluated the prevalence of aflatoxins (AFB1, AFB2, AFG1, and AFG2) in maize sold across 13 Local Government Areas (LGAs) in Nasarawa State, Nigeria, and examined how environmental factors contribute to fungal growth and mycotoxin production. Maize samples (130) were subjected to total heterotrophic fungal count (THFC) analysis using standard microbiological methods, while aflatoxin levels were measured using thin-layer chromatography (TLC) combined with densitometry. Statistical methods, including analysis of variance (ANOVA) and regression modeling, were utilized to clarify spatial contamination trends and identify environmental variables that could predict contamination. The findings revealed significant aflatoxin contamination, with 85.4% of samples surpassing the 20-ppb safety limit set by the Standards Organisation of Nigeria (SON). Aspergillus flavus, the main producer of aflatoxin B1, was found in 94% of the samples, followed by Fusarium verticillioides (71%) and Aspergillus niger (59%). Regression analysis showed a strong correlation (r = 0.710, P<0.001) between fungal load and AFB1 levels, with humidity explaining 41.1% of the variability in THFC. The highest AFB1 concentration (137.10 ± 15.10 ppb) was found in Doma, while Lafia showed consistently lower contamination levels, likely due to better post-harvest handling practices. This study underscores the urgent need for targeted interventions to reduce aflatoxin contamination, such as rapid drying, hermetic storage systems, and educating farmers on preventing fungal growth. It also recommends implementing aflatoxin surveillance programs and researching resistant maize varieties to improve food safety and public health in Nasarawa State.

Dimensions

REFERENCES

Ranum, P., Peña-Rosas, J. P., & Garcia-Casal, M. N. (2014). Global maize production, utilization, and consumption. Annals of the New York Academy of Sciences, 1312(1), 105–112. https://doi.org/10.1111/nyas.12396

Rouf Shah, T., Prasad, K., & Kumar, P. (2016). Maize, a potential source of human nutrition and health: A review. Cogent Food & Agriculture, 2(1), Article 1166995. https://doi.org/10.1080/23311932.2016.1166995

Galani, Y. J. H., Ligowe, I. S., Kieffer, M., Kamalongo, D., Kambwiri, A. M., Kuwali, P., ... & Orfila, C. (2022). Conservation agriculture affects grain and nutrient yields of maize (Zea mays L.) and can impact food and nutrition security in Sub-Saharan Africa. Frontiers in Nutrition, 8, Article 804663. https://doi.org/10.3389/fnut.2021.804663

FAO. (2019). Global Information and Early Warning System (GIEWS). Food and Agriculture Organization of the United Nations. https://www.fao.org/faostat/en/#home

Mang’eni, F. O. (2022). Historical analysis of declining maize production in Kenya: A case of Trans-Nzoia County. Asian Research Journal of Arts & Social Sciences, 5(7), 312–316.

Dabija, A., Ciocan, M. E., Chetrariu, A., & Codină, G. G. (2022). Buckwheat and amaranth as raw materials for brewing: A review. Plants, 11(6), Article 60756. https://doi.org/10.3390/plants11060756

Ogara, I., Zarafi, B., Alabi, O., Banwo, O., Ezekiel, C. N., Warth, B., ... & Krska, R. (2017). Mycotoxin patterns in ear rot infected maize: A comprehensive case study in Nigeria. Food Control, 73, 1159–1168. https://doi.org/10.1016/j.foodcont.2016.10.034

Akharenegbe, P., Chuku, A., Mawak, J., Sani, B., Nsemoh, H. E., & Fadayomi, V. K. (2022). Fungal biodiversity associated with groundnuts stored in Nasarawa State. GSC Biological and Pharmaceutical Sciences, 18(3), 023–029. https://doi.org/10.30574/gscbps.2022.18.3.0087

Zheng, Y., Wu, W., Sun, C., Liu, H., & Dou, J. (2024). Occurrence and fate analysis of mycotoxins in maize during the post-harvest period. Toxins, 16, Article 459. https://doi.org/10.3390/toxins16110459

Chang, W. L., Saad, H. A., Jamaluddin, R., & Sabran, M. R. (2023). Aflatoxin occurrence, food regulations, dietary exposure, and risk assessment: A mini-review from the Malaysian perspective. Malaysian Journal of Medicine and Health Sciences, 19(1), 296–306. https://doi.org/10.47836/mjmhs.19.1.38

PACA. (2018). Country-led aflatoxin and food safety situation analysis and action planning for Nigeria: Final report. Partnership for Aflatoxin Control in Africa, African Union Commission.

Batagarawa, U. S., Dangora, D. B., & Haruna, M. (2015). Aflatoxin contamination in some selected grains, feeds and feed ingredients in Katsina and Zaria Metropolis. Annals of Experimental Biology, 3(3), 1–7. http://www.aexpbio.com/

Abdurrazaq, M., Tijjani, M. B., & Atta, H. I. (2022). Assessment of aflatoxin contamination in maize and groundnuts during storage in Giwa community, Kaduna State Nigeria. UMYU Journal of Microbiology Research, 7(2), E-ISSN: 2814–1822; P-ISSN: 2616–066. https://doi.org/10.47430/ujmr.2272.004

Chuku, A., Arikpo, G., Obande, G., Akharenegbe, P., Uteh, P. U., & Namang, M. (2016). Mycological assessment of the air quality in flood-prone homes within Lafia Local Government Area of Nasarawa State. FULafia Journal of Science and Technology, 2(1), 1–10.

Sobolev, V. S., & Dorner, J. W. (2002). Cleanup procedure for determination of aflatoxins in major agricultural commodities by liquid chromatography. Journal of AOAC International, 85(3), 642–645. https://doi.org/10.1093/jaoac/85.3.642

Atehnkeng, J., Ojiambo, P. S., Donner, M., Ikotun, T., Sikora, R. A., Cotty, P. J., & Bandyopadhyay, R. (2008). Distribution and toxigenicity of Aspergillus species isolated from maize kernels from three agro-ecological zones in Nigeria. International Journal of Food Microbiology, 122(1–2), 74–84. https://doi.org/10.1016/j.ijfoodmicro.2007.11.062

Cotty, P. J., & Cardwell, K. F. (1999). Divergence of West African and North American communities of Aspergillus section Flavi. Applied Environmental Microbiology, 65(5), 2264–2266. https://doi.org/10.1128/AEM.65.5.2264-2266.1999

Soba, T. M., Clement, S. A., Ndagi, H. I., Obadiah, C., & Shammasu, L. D. (2023). Abundance and diversity of fungi under three tree species in Shabu-Lafia, Nasarawa State, Nigeria. Journal of Research in Forestry, Wildlife and Environment, 15(2), 19–30.

Blacutt, A. A., Gold, S. E., Voss, K. A., Gao, M., & Glenn, A. E. (2018). Fusarium verticillioides: Advancements in understanding the toxicity, virulence, and niche adaptations of a model mycotoxigenic pathogen of maize. Phytopathology, 108(3), 312–326. https://doi.org/10.1094/PHYTO-06-17-0203-RVW

Klich, M. A. (2007). Aspergillus flavus: The major producer of aflatoxin. Molecular Plant Pathology, 8(6), 713–722. https://doi.org/10.1111/j.1364-3703.2007.00436.x

Magnussen, A., & Parsi, M. A. (2013). Aflatoxins, hepatocellular carcinoma, and public health. World Journal of Gastroenterology, 19(10), 1508–1521. https://doi.org/10.3748/wjg.v19.i10.1508

Patil, G., Keche, A., & Madkey, M. (2024). Mycotoxicoses in humans. IntechOpen. https://doi.org/10.5772/intechopen.1006944

Chidimma, E., Tasie, F. O., & Mbah-Omeje, K. N. (2024). Identification and characterization of multi-mycotoxins recovered from dry corn in selected markets in Enugu Metropolis, Enugu State, Nigeria. South Asian Journal of Research in Microbiology, 18(9), 30–50. https://doi.org/10.9734/sajrm/2024/v18i9386

Shabeer, S., Asad, S., Jamal, A., & Ali, A. (2022). Aflatoxin contamination, its impact, and management strategies: An updated review. Toxins, 14(5), Article 307. https://doi.org/10.3390/toxins14050307

Dedesko, S., & Siegel, J. A. (2015). Moisture parameters and fungal communities associated with gypsum drywall in buildings. Microbiome, 3(1), Article 71. https://doi.org/10.1186/s40168-015-0137-y

Waheed, A., Haxim, Y., Islam, W., Ahmad, M., Muhammad, M., Alqahtani, F. M., ... & Zhang, D. (2023). Climate change reshaping plant-fungal interaction. Environmental Research, Article 117282. https://doi.org/10.1016/j.envres.2023.117282

Liang, H., Yang, M., Li, Q., Zhang, L., & Zhao, X. (2024). A comprehensive review of the main components of plant essential oils and the mechanisms responsible for the inhibitory effects on fungal growth and aflatoxin synthesis. Innovative Food Science & Emerging Technologies, 103, Article 103747. https://doi.org/10.1016/j.ifset.2024.103747

Oyebamiji, Y. O., Adebayo, I., Umar, O. B., Mohd Zaini, N. A., & Ismail, M. N. (2024). Occurrence, health implications, and management of aflatoxin in cereal: A current review. Egyptian Journal of Botany, 64(4), 1–15. https://doi.org/10.21608/ejbo.2023.196133.2265

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Published

2025-01-09

How to Cite

Prevalence and Environmental Determinants of Aflatoxin Contamination in Maize Sold in Open Markets Across Nasarawa State, Nigeria. (2025). Lafia Journal of Scientific and Industrial Research, 3(1), 47-54. https://doi.org/10.62050/ljsir2025.v3n1.391