The Effect of Post-treatment with Yellow Bitter Yam (Dioscorea dumetorum) on the Antioxidant Status of Streptozocin-induced Diabetic Rats

Esther Mayowa Pius; Olubunmi Bolanle Ajayi; Abubakar Mohammed; Timothy Atinuke; Olofin Opeyemi; Amina Ladidi Musa

doi:10.62050/ljsir2024.v2n1.264

المؤلفون

Esther Mayowa Pius
piusmayowaesther@gmail.com

Department of Biochemistry, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria
https://orcid.org/0009-0007-0524-592X
Olubunmi Bolanle Ajayi
Department of Biochemistry, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria
Abubakar Mohammed
Department of Biochemistry, Federal University of Lafia, Nasarawa State, Nigeria
Timothy Atinuke
Department of Biochemistry, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria
Olofin Opeyemi
Department of Biochemistry, Ekiti State University, Ado-Ekiti, Ekiti State, Nigeria
Amina Ladidi Musa
Department of Biochemistry, Federal University of Lafia, Nasarawa State, Nigeria

الكلمات المفتاحية:

Array، Array، Array، Array، Array

الملخص

This study assesses the effects of supplemented diet of Discorea dumenturom on antioxidant status of streptozotocin-induced diabetic rats. Twenty-five adult male wistar rats were randomly assigned into five groups each containing five rats, group two to five were subjected to high fat diet for four weeks and was thereafter induced with STZ intraperitoneally (45mg/kg body weight) resulting to type 2 diabetes. The body weight, glucose concentration and the activities the antioxidant enzymes, Glutathione peroxidase (GPx), Glutathione S-transferase (GST), Catalase (CAT), and Malondialdehyde (MDA) and Glutathione concentration in the kidney, heart and liver were assessed. The Post-treatment with 10% and 20% Yellow Bitter Yam significantly reduced glucose and an healthy reduction in weight in treated groups compared to diabetic controls. In diabetic kidneys, GSH and GST were similar to controls, while GPX, CAT, and MDA decreased. Diabetic hearts showed increased CAT, GSH, and MDA, regulated in the treated group. In diabetic livers, GPx, GST, and MDA were consistent across groups, but GSH and CAT significantly (p>0.05) decreased in post-treated groups. The study suggests Dioscorea dumetorum supplementation regulates antioxidant enzymes, indicating potential hypoglycemic and antioxidative effects, particularly in kidney, heart, and liver enzyme activities associated with Type 2 diabetes.

Dimensions

REFERENCES

World Health Organization study group on diabetes mellitus, (2021). Technical report series No. 844, World Health Organization, Geneva, pp. 78 – 79.

IDF (2021). Diabetes Atlas. 15th Edition, International Diabetes Federation (IDF). www.diabetesatlas.org

Ray, S., Nagarchi, L., Das, I., Achuthananthan, J. and Krishnamurthy, S. (2015). Cytotoxicity, genotoxicity, and phytotoxicity of tannery effluent discharged into Palar River Basin, Tamil Nadu, India. Journal of Toxicology, 1-9. https://doi.org/10.1155/2015/504360

Adeloye, D., Ige, J. O., Aderemi, A. V., Adeleye, N., Amoo, E. O. and AsaAuta, G. O. (2017). Estimating the prevalence, hospitalization and mortality from Type 2 diabetes mellitus in Nigeria: A systematic review and meta-analysis. BMJ Open 11, 7(5). https://doi.org/10.1136/bmjopen-2016-015424

Nestor, K. K., Goerge, G. T., Jacko, F. A., Massara, C. C. and Goerges, N. A. (2009). Influences of the variety and cooking method on Glycemic index of yam. J. Nutr., 8, 993-999.

Kumar, S. and Jena, P. K. (2017). Tools from biodiversity: Identifying frontier research integrating mathematic approaches to diverse systems/sustainability. Journal of Wild Nutraceutical Plants, 54(5), 89-94. https://doi.org/10.1007/978-3-319-43901-3-9

Kumar, S, Mahanti, P, Singh, N. R., Rath, S. K., Jena, P. K. and Patra, J. K. (2017). Antioxidant activity, antibacterial potential, and characterization of active fraction of Dioscorea pentaphylla L. tuber extract collected from Similipal Biosphere Reserve, Odisha, India. Brazilian Journal of Pharmaceutical Sciences, 53(4), e170006.

Swain, J., Kumar, S., Parida, S. and Jena, P. K. (2020). In-vitro screening of antibacterial activities of selected wild tuberous plants of Odisha, India. Asian J. of Biol. and Life Sci., 9(1), 79-87.

Ukom, A. N., Ojimelukwe, P. C., Ezeama, C. F., Ortiz, D. O. and Aragon, I. (2014). Phenolic content and antioxidant activity of some under-utilized Nigerian yam (Dioscorea spp.) and cocoyam (Xanthosomamaffa (Scoth)) tubers. IOSR J. of Envtal Sci., Toxicology and Food Techn., 8, 104–111.

Iwu, M. M., Okunji, C. O., Ohiseri, G. O., Akah, P., Corley, D. and Tempesta, M. S. (1990). Hypoglycaemic activity of dioscoretine from tubers of Dioscorea dumetorum in normal and alloxan diabetic rabbits. Planta Medica, 56, 264-267.

Price, E. J., Wilkin, P., Sarasan, V. and Fraser, P. D. (2016). Metabolite profiling of Dioscorea (yam) species reveals underutilised biodiversity and renewable sources for high-value compounds. Scientific Reports, 6: 29136.

Sonibare, M. A. and Abegunde, R. B. (2012). In vitro antimicrobial and antioxidant analysis of Dioscorea dumetorum (Kunth.) Pax and Dioscorea hirtiflora (Linn.) and their bioactive metabolites from Nigeria. J. of Applied Biosci., 51, 3583–3590.

Malviya, N., Jain, S. and Malviya, S. (2010). Antidiabetic potential of medicinal plants. Acta Poloniae Pharmaceutica – Drug Research, 67, 113–118.

Egbuonu, A. C. C., Nzewi, D. C. and Egbuonu, O. N. C. (2014). Functional properties of bitter yam (Dioscorea dumetorum) as influenced by soaking prior to oven-drying. Ameri. J. of Food Techn., 9, 97-103.

Nimenibo-Uadia, R. I. and Oriakhi, A. V. (2017). Proximate, Mineral and Phytochemical composition of Dioscorea dumetorum. Pax. J. Appl. Sci. Environ., 21 (4), 771-774.

Mounien, L., Tourniaire, F. and Landrier, J. F. (2019). Anti-obesity effect of carotenoid: Direct impact on adipose tissue and adipose tissue-driven indirect effects. Nutrients, 11, 1562.

Rodriguez-Concepeion, M., Avalos, J., Bonet, M. L., Boronat, A., Lourdes, G. G., Hornero-Mendez, D., Singh, S. K., Kesari, A. N., Gupta, R. K, Jaiswal, D. and Watal, G. (2007). Assessment of antidiabetic potential of Cynodondactylon extract in streptozotocin diabetic rats. Journal of Ethnopharmacology, 1142, 174-179.

Ochei, J. and Kolhatkar, A. (2000). Lipids and lipoprotein. In: Medical Laboratory Science: Theory and Practice. McGraw Hill Publishing Co. Ltd., New Delhi, pp. 311-347.

Varshney, R. and Kale, R. K., (1990). Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int. J. of Radiation Bio., 58, 733-43.

Szkudelski, T. (2012). Streptozotocin–nicotinamide induced diabetes in the rat: Characteristics of the experimental model. Exp. Biol. Med., 237(5), 481-490.

Nagarchi, K., Ahmed, S., Sabus, A. and Saheb, S. H. (2015). Effect of streptozotocin on glucose levels in albino Wistar rats. Journal of Pharmaceutical Sci. and Res., 7(2), 67-69.

Matsunami, T., Sato, Y., Hasegawa, Y., Ariga, S., Kashimura, H., Sato, T. and Yukawa, M. (2011). Enhancement of reactive oxygen species and induction of apoptosis in streptozotocin- induced diabetic rats under hyperbaric oxygen exposure. Int. J. of Clinical and Experimental Pathol., 4(3), 255.

Abolfathi, A. L., Mohajeri, D., Rezaie, A. and Nazeri, M. (2012). Protective effects of green tea extract against hepatic tissue injury in streptozotocin-induced diabetic rats. Evidence-Based Complementary and Alternative Medicine: Article ID 740671, 10 pages. https://doi.org/10.1155/2012/740671

Hermansen, K. and Montensen, L. S. (2007). Body weight changes associated with antihyperglycaemic agents in type 2 diabetes mellitus. Drug Safety, 30, 1127-1142. https://doi.org/10.2165/00002018-200730120-00005

Faivaz Ahmed and AsnaUroo, J. (2009). Total phenolic content and antioxidant activity of aqueous and methanol extracts of Dioscareaalata L. tuber. Journal of Pharmacy Research, 2(10):1, 663-1665.

ingh, S. R., Ahmad, F., Lal, A., Yu, C., Bai, Z. and Bennett, H. (2007). Efficacy and safety of insulin analogues for the management of diabetes mellitus: Ameta-analysis. CMAJ, 180(4), 385- 397. https://doi.org/10.1503/cmaj.081041

Jiang, B., Guo, L. and Li, B. Y. (2013). Resveratol attenuates early diabetic nephropathy by down regulating gluthathione S-transferase Mu in diabetic rats. Journal of Medicinal Food, 16, 481- 486.

Noce, A., Fabrini, R., Dessi, M, et al. (2014). Erythrocyte glutathione transferase activity: A possible early biomarker for blood toxicity in uremic diabetic patients. Acta Diabetologica, 51, 219-224.

Okutan, H. N., Ozcelik, H., Yilmaz, R. and Uz, E. (2005). Effects of caffeic acid phenethyl ester on lipid peroxidation and antioxidant enzymes. Clinical Biochemistry, 38(2), 191-198.