PHENOTYPIC DETECTION OF PLASMID FROM CIPROFLOXACIN RESISTANT SALMONELLA TYPHI ISOLATED FROM STOOL OF NSUK STUDENTS

Authors

  • Usman Shuaibu Okposhi
    Federal University of Lafia
  • Umar Saleh Aliyu
    National Institute for Freshwater Fisheries Reseatrch, New Bussa, Niger State, Nigeria
  • Abdulaziz Dardau Aliyu
    Federal University of Lafia
  • Isah Ibrahim Okposhi
    Federal University of Lafia
  • Danteni Dauda Odonye
    Department of Microbiology, Federal University of Lafia
  • Fatima Yusuf Almustapha
    Department of Microbiology, Federal University of Lafia
  • Kabiru Abdullahi Shuaibu
    Department of Microbiology, Federal University of Lafia

Keywords:

Phenotypic , Salmonella-typhi , Plasmid , Ciprofloxacin , Resistant , Stool

Abstract

Salmonella typhi (S. typhi) is a common cause of morbidity and mortality in developing countries. Studies on
phenotypic detection of plasmid from ciprofloxacin resistant S. typhi isolated from stool of new students Nasarawa
State University, Keffi, was carried out. A total of 180 stool samples were collected from the new students. 30 stool
samples were collected from each of the six respective Faculties. 29(16.1%) Salmonella isolates were recovered and
identified by standard Microbiological methods. 21(72.4%) of the isolates were Salmonella typhi and 8(27.6%)
were Salmonella enteritidis. The prevalence of Salmonella typhi isolation with respect to Faculties was highest in
the Faculties of Art (ART) and Law (LAW) with 6(28.6%) isolates each. On the basis of gender, 15 samples each
were collected from both male and female students respectively and the highest prevalence of isolation was 4(8.0%)
amongst males in the Faculty of LAW and females in the Faculty of ART, while the least prevalence of isolation
was zero (0) amongst males in the Faculty of NAT. On the bases of age, the prevalence of isolation was highest
amongst the age group 21-30, 19(10.5%), while age group 51-60 showed zero (0) prevalence of isolation.
Antimicrobial Susceptibility was carried out using CLSI method; and 21(72.4%) Salmonella typhi isolates were
susceptible to Ciprofloxacin, while 8(27.6%) were resistant and the antimicrobial resistance phenotypic pattern of
the isolates was also determined. The ciprofloxacin resistance isolates were subjected to “Curing Method” to
determine if the resistance was plasmid-mediated or not.

 

Author Biographies

Usman Shuaibu Okposhi

Department of Microbiology, Lecturer II

Isah Ibrahim Okposhi

Department of Science Laboratory Technology, Assistant Lecturer 

Dimensions

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Published

24-08-2024

How to Cite

PHENOTYPIC DETECTION OF PLASMID FROM CIPROFLOXACIN RESISTANT SALMONELLA TYPHI ISOLATED FROM STOOL OF NSUK STUDENTS. (2024). FULafia Journal of Science and Technology , 8(2), 17-22. https://doi.org/10.62050/fjst2024.v8n2.280

Issue

Vol. 8 No. 2 (September, 2024): Fulafia Journal of Science and Technology (FJST)

Section

Agricultural and Biological Sciences
Copyright & Licensing

How to Cite

PHENOTYPIC DETECTION OF PLASMID FROM CIPROFLOXACIN RESISTANT SALMONELLA TYPHI ISOLATED FROM STOOL OF NSUK STUDENTS. (2024). FULafia Journal of Science and Technology , 8(2), 17-22. https://doi.org/10.62050/fjst2024.v8n2.280

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